Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes.
نویسندگان
چکیده
In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 39 1 شماره
صفحات -
تاریخ انتشار 2001